Endurance training alters motor unit activation strategies for the vastus lateralis, yet sex-related differences and relationships with muscle size remain

Purpose: To examine the effects of 10 weeks of endurance cycling training on mechanomyographic amplitude (MMG_RMS)–torque relationships and muscle cross-sectional area (mCSA) of the vastus lateralis (VL) for 10 sedentary males (Age ± SD; 20.2 ± 1.9 years) and 14 sedentary females (21.9 ± 5.3 years). Methods: Participants performed maximal voluntary contractions (MVCs) and an isometric ramp up muscle action to 70% MVC of the knee extensors before (PRE) and after training at the same absolute pre-treatment submaximal torque (POST_ABS). MMG was recorded from the VL and b terms were calculated from the natural log-transformed MMG_RMS–torque relationships for each subject. mCSA was determined with ultrasonography. Results: Cycling decreased MVCs from pre- (168.10 ± 58.49 Nm) to post-training (160.78 ± 58.39 Nm; p = 0.005) without changes in mCSA. The b terms were greater for POST_ABS (0.623 ± 0.204) than PRE (0.540 ± 0.226; p = 0.012) and for males (0.717 ± 0.171) than females (0.484 ± 0.168; p = 0.003). mCSA was correlated with the b terms for PRE (p < 0.001, r = 0.674) and POST_ABS (p = 0.020, r = 0.471). Conclusion: The decrease in MVC and increase in MMG_RMS (b terms) post-training suggests increased motor unit (MU) recruitment to match pre-training torques. The greater acceleration in the b terms by males may reflect sex-related differences in fiber-type area. MMG_RMS–torque relationships during a high-intensity contraction provided insight on MU activation strategies following endurance training and between sexes. Furthermore, the findings suggest a relationship between MMG_RMS and muscle size.