Evidence that the yeast spindle assembly checkpoint has a target other than the anaphase promoting complex
- Catherine A. Andrewsb(Author),
- ,
- Juan F. Giménez-Abiánb, c(Author),
- Vincent Guaccia(Author),
- Amit C. Vasb(Author),
- Duncan J. Clarkeb(Author)
- aFox Chase Cancer Center,
- bUniversity of Minnesota Medical School,
- cCSIC - Centro de Investigaciones Biológicas Margarita Salas (CIB)
Research Output: Contribution to journal Article Peer-review
Open access
Abstract
The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G2/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset.
Publication metrics
PlumX, opens in new tab
Captures
16
Citations
9